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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Mol Biosyst. 2010 Oct 8;7(1):74–81. doi: 10.1039/c0mb00076k

Figure 5.

Figure 5

Tandem purification to get different PhDph2 dimers. a. Diagram showing the tandem purification strategy to get different PhDph2 dimers; b. Tandem purification of PhDph2 dimers: left, elutions from Ni-NTA purification by using different concentrations of imidazole solutions to get PhDph2 (WT-His6) and PhDph2 (DM-GST: WT-His6) mixture; Right, purification of PhDph2 (DM-GST), PhDph2 (DM-GST: WT-His6), and PhDph2 (WT-His6). PhDph2 (WT-His6). c. Purified phDph2 dimers. The PhDph2 (WT-His6) shown here was further purified by heating at 95 °C.