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Activity assay of PhDph2 heterodimer. a. 14C-labeling of PhEF2 catalyzed by PhDph2 (WT-His6), PhDph2 (DM-GST: WT-His6), PhDph2 (DM: WT-His6), and PhDph2 (DM-GST). The PhDph2 (DM: WT-His6) was obtained by TEV digest of PhDph2 (DM-GST: WT-His6) to remove the GST tag. The left panel shows the Coomassie blue-stained gel and the right panel shows the autoradiography. b. The formation of MTA was detected by HPLC.
