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(a) MECs from β1fx/fx;CreER™ mice were cultured on 3D BM-matrix for 3-d in the presence or absence of 4OHT, isolated using PBS-EDTA and replated onto collagen I coated plates for 2-d or 3-d, where they formed a cell monolayer. These cells have lost β1-integrin expression, shown by immunoblotting. (b) The integrin-deleted cells showed defective proliferation by EdU incorporation.
