Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Mar 29;6(3):e18247. doi: 10.1371/journal.pone.0018247

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Fukuma et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Both RD-114 vector (100 ng) and the expression vector for human or feline Tetherin/BST-2 containing FLAG-tag (25, 50, 100, or 200 ng) were cotransfected into 293T cells. Cells and viruses were collected at 48 h after transfection, and analyzed by Western blotting (A). The intensities of the bands for virus- and cell-associated Gag were quantified using a Fuji LAS3000 imaging system (Fuji Film) (B). The control vector (virus p28^CA/virus p28^CA + cellular p28^CA + p68^Gag) was set to 100%. Histograms represent the averages from three independent experiments (± standard deviation of the mean). (C) RD-114 viral RNA in the supernatant from cells was quantified by real-time RT-PCR.