Table 1. Oligonucleotide primers used in this study and estimated size of PCR products.

Name of primers	Sequences (5′ to 3')
genoE2 SS	TGCCCATAGGTTGTCAGACT
genoNEO SS	TCACCTTAATATGCGAAGTGG
genoE2 AS	ATGTGCTAACCATGACCTTGA
genoE3 rev	GAGAGACCTCAGATGGTGGAG
CreSS	CCGGGCTGCCACGACCAA
CreAS	GGCGCGGCAACACCATTTTT
SA-4400SpeI	ACTAGTTGCTTCACCTCTTCAAAGTTTC
SA-6400SalI	GTCGACTTTCAGCCTGTACATCCTGTGC
E2-6400SpeI	ACTAGTGATCAGAAGCTCAAGGTCATGG
E2-7308KpnI	GGTACCCTTCCAGAATTGCAGGTAACTG
E2-5probeSS	TGCCCAGAACTACTGTAAAGC
E2-5probeAS	TGCTGTTTAAAGCATCTCTGC
E2-3probeSS	GGGTAGAGCAATGGGTGTATT
E2-3probeAS	AGAATGCACGTCGTCTTGTAG

The oligonucleotide sequences used in this study are listed in the upper table. The oligonucleotide primers were used to genotype the mice, to obtain genomic fragments of Snap23, and to generate probes for Southern blot analysis. The estimated sizes of PCR products obtained during genotyping the mice are indicated in the lower table.