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. 2011 Mar 29;6(3):e18356. doi: 10.1371/journal.pone.0018356

Figure 2. Cytotoxicity and secretion profiles of PA103ΔpcrV host strain complemented with pcrV::EZ-linker constructs.

Figure 2

(A) LDH release from HeLa cells as a quantitative measurement of cytotoxicity during infection. Cell culture supernatants were assayed for LDH activity in triplicate of two independent experiments for statistical analyses. Representative strains are shown. Error bars indicate SD. (B) Secretory regulation profiles of PcrV derivatives. Bacterial cells were grown to suppress (−NTA) type III secretion and culture supernatants were subjected to Western blot analyses to quantify ExoU release. Deregulated ExoU secretion (highlighted by boxes) was quantified based on the amount of constitutively secreted ExoU by PA103ΔpcrV vector control (pUCP) as 100%. Deregulated/cytotoxic and deregulated/noncytotoxic phenotypes were shown as dotted and gray boxes, respectively. Results are representative of at least three independent experiments.