| PA103 |
Wild-type cytotoxic strain, expresses type III proteins and translocates effectors, ExoU and ExoT. |
| PA103+pUCP-pS-pcrV
|
Wild-type strain harboring a plasmid copy of pcrV under control of the type III exoS promoter. Cytotoxic. |
| PA103exsAΩ+pUCP |
Type III knockout strain, mutation in the type III regulatory gene, exsA, prevents activation of the type III secretion system. Noncytotoxic. |
| PA103ΔpcrV+pUCP (PA103ΔV+pUCP) |
Nonpolar pcrV deletion harboring a plasmid. Noncytotoxic and constitutive secretion. |
| PA103ΔpcrV+pUCP-pS-pcrV(PA103ΔV+pcrV) |
Complemented mutant harboring a plasmid copy of pcrV under control of the type III exoS promoter. Cytotoxic. |
| PA103ΔV+pUCP-pS-exoUS142A(PA103ΔV+exoU-S142A) |
Nonpolar pcrV mutant harboring a plasmid copy of exoU with a point mutation at its catalytic site. Noncytotoxic and constitutive secretion. |
| PA103ΔpopB (PA103ΔB) |
Nonpolar popB deletion. Noncytotoxic. |
| PA103ΔpopB+pUCP-pS-popB(PA103ΔB+popB) |
Complemented mutant harboring a plasmid copy of popB. Cytotoxic. |
| PA103ΔUT+pUCP(PA103ΔUT+pUCP) |
ExoU and ExoT knockout strain harboring a plasmid. Regulated secretion and noncytotoxic. |
| PA103ΔUT+pUCP-pS-exoUS142A(PA103ΔUT+exoU-S142A) |
ΔUT strain harboring a plasmid copy of exoU with a point mutation at its catalytic site. Regulated secretion and noncytotoxic. |
| PA103ΔpcrV+pUCP-pS-pcrV-EZ(PA103ΔV+EZ)EZ16, EZ28, EZ77, EZ96, EZ101, EZ104, EZ105, EZ107, EZ109, EZ113, EZ116, EZ154, EZ155, EZ179, EZ180, EZ198, EZ202, EZ204, EZ214 |
Nonpolar pcrV mutant harboring a plasmid copy of pcrV::EZ linker.Regulated secretion and cytotoxic. |
| PA103ΔV+EZ44, EZ52, EZ64 |
Deregulated secretion and cytotoxic. |
| PA103ΔV+EZ119, EZ120, EZ131, EZ134, EZ138, EZ279 |
Deregulated secretion and noncytotoxic. |
| PA103ΔpcrV+pUCP-pS-pcrV-L63A or D133A (PA103ΔV+L63A or D133A) |
Nonpolar pcrV mutant harboring a plasmid copy of pcrV with a point mutation at amino acid residue 63 or 133. Regulated secretion with delayed cytotoxicity. |
| PA103+pUCP-pS-pcrV-EZ (PA103+EZ) EZ44, EZ52, EZ64, EZ119, EZ120, EZ131, EZ134, EZ138, EZ279 |
Wild-type strain harboring a plasmid copy of pcrV::EZ-linker.Regulated secretion and cytotoxic. |
| PA103+pUCP-pS-pcrV-L63A or D133A(PA103+L63A or D133A) |
Wild-type strain harboring a plasmid copy of pcrV with a point mutation at amino acid residue 63 or 133. Regulated secretion and cytotoxic. |
