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. 1995 Jan 11;23(1):36–41. doi: 10.1093/nar/23.1.36

Allele-specific methylation and expression of an imprinted U2af1-rs1 (SP2) gene.

I Hatada 1, K Kitagawa 1, T Yamaoka 1, X Wang 1, Y Arai 1, K Hashido 1, S Ohishi 1, J Masuda 1, J Ogata 1, T Mukai 1
PMCID: PMC306627  PMID: 7870588

Abstract

The mouse U2af1-rs1(SP2) gene, which was cloned by a two-dimensional genome scanning method, is expressed exclusively from the paternally inherited chromosome. This gene has significant similarity to U2AF and located in chromosome 11, of which maternal duplication/paternal deficiency results in a small body. In this report, we cloned genomic U2af1-rs1(SP2) and found its promoter was methylated in a maternal-allele-specific manner. This allelic methylation was not established in parental gametes, but established between 1.5 d.p.c. and 12.5 d.p.c. on the contrary, the allele-specific expression occurred in the two-cell stage when transcription initiates. Absence of the methylation of the upstream region in this stage indicates that methylation is not necessary for inactivation of the expression.

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Selected References

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