. Author manuscript; available in PMC: 2011 Oct 1.

Published in final edited form as: Nat Neurosci. 2011 Mar 13;14(4):469–477. doi: 10.1038/nn.2781

Table I.

Tyramine uptake competition assay.

	Tyramine K_M (µM)	V_MAX (%)
Flot1 WT	2.30 [1.25 – 4.22]	100
Flot1(C34A)	2.17 [1.19 – 3.19]	95 ± 3.2

siCTRL	2.73 [1.99 – 3.74]	100
siFlot1	2.69 [1.67 – 4.32]	91 ± 24

Ctrl	6.04 [5.27 – 6.92]	100
Nys	5.86 [5.00 – 6.76]	103 ± 6.6

The Km values were calculated from the observed IC50 value found by non-linear regression analysis of [³H] tyramine uptake competition assays. Data obtained from EM4 cells transiently expressing YFP-DAT. Data are means + SEM of 2–5 experiments performed in duplicate. The SEM interval for each Km value is indicated in brackets and was calculated from the pKI + SEM The calculated Vmax for [³H]tyramine uptake was normalized to the respective control values after normalization for cell density.