Figure 1. Brca2 inactivation promotes pancreatic cancer when combined with Trp53 inactivation.

(A) Schematic representation of the Brca2^F11 allele and the Trp53^F2-10 allele before and after pdx-1-cre dependent recombination. (B) PCR analysis demonstrating rearrangement of the Brca2 and Trp53 alleles in response to pdx-1-cre expression using DNA from mouse tail and pancreas. (C) Kaplan-Meier plots showing pancreatic cancer free survival of aged CPB2^Δ11/Δ11 (n=47), CPB2^wt/Δ11 (n=41), and CPB2^wt/wt (n=34) mice. p- values were determined by a log rank test. (D) Frequency of histological subtypes of tumors detected in CPB2^Δ11/Δ11, CPB2^wt/Δ11 and CPB2^wt/wt mice. (E) Representative images from tumors derived from CPB2^Δ11/Δ11 mice stained with H&E (i,iv,vii,x), cytokeratin 19 (ii,v,viii,xi) or amylase (iii,vi,ix,xii). (F) PanIN lesions (i,ii) and multinucleated cells (iii) from a CPB2^Δ11/Δ11 pancreas.