Table I.

Morphological and Biochemical Characteristics of Bacillus subtilis KIBGE HAS

Parameters	Characteristics
Morphology	Rods with rounded ends. Finely wrinkled, dull, opaque, adherent colonies on nutrient agar plate.
Gram’s reaction	Positive
Growth factors
Oxygen	Strict aerobe
Temperature	Growth at 35°C, range, 25–45°C
pH	Optimum 7.0, range, 5.0–9.0
Biochemical reaction
Indole	Positive
Methyl Red	Negative
Vogues Prosker	Positive
Citrate	Utilized
Starch hydrolysis	Positive
Nitrite	Formed from nitrate
Fermentation reaction
Glucose, fructose, galactose, lactose and sucrose	Acid