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Steps Involved for the Purification of α-Amylase from Bacillus subtilis KIBGE HAS
| Purification steps | Enzyme activity (units) | Total protein (mg) | Specific activity (U/mg) | Fold purification |
|---|---|---|---|---|
| Crude enzyme | 684000 | 5063 | 135.0 | 1.0 |
| Ultrafiltration | 141000 | 308 | 457 | 3.4 |
| Ammonium Sulfate precipitation | 47405 | 18.3 | 2590 | 19.1 |
| Dialysis | 41080 | 14.6 | 2813 | 20.8 |
| Sepharose CL-6B | 38385 | 2.95 | 13011 | 96.3 |
