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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Gastroenterology. 2011 Jan 14;140(4):1230–1240.e7. doi: 10.1053/j.gastro.2011.01.005

Figure5. Loss of intestinal stem cell markers in Notch signaling deficient Dll1-Dll4 and RBP-J mutant mice.

Figure5

(A) In situ hybridization analysis for SC markers Lgr5, Olfm4 and Ascl2 on sections of the small intestine. Simultaneous inactivation of Dll1 and Dll4 or RBP-J resulted in the loss of expression of all three SC markers described above. Insets show high magnification (400x) of outlined crypt regions. Note that for Olfm4 in situ hybridization on Dll1-Dll4 mutant mice a region with a few positive SC (presumably none deleted) was chosen on purpose to indicate successful probe labeling.