Figure6. Klf4 is dispensable for goblet cell metaplasia induced by loss of Notch signaling.
(A-B) Alcian blue (top panels) and Ki67 (bottom panels) staining on sections derived from the proximal intestine (A) and colon (B) of the indicated mice. Note the complete loss of the proliferative crypt compartment and the complete conversion into goblet cells in RBP-J and RBP-J Klf4 mutant intestine. (C) Pharmaceutical inhibition of Notch signaling using the γ-secretase inhibitor DBZ after gut specific inactivation of Klf4 results in the loss of proliferating crypt progenitors due to their conversion into postmitotic goblet cells.