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Detection limit of egg white allergen. RS-ATL8 cells were sensitized with 1 : 100-diluted healthy donor's serum (open circles) or egg allergy patient serum (closed circles) overnight. Cells were stimulated with the indicated concentrations of egg white proteins (EWP) diluted in 10% Fetal calf serum-containing medium for 3 h. Dashed line, two-fold level of background (0 g/ml EWP) luciferase expression. Data are means ± SEM (n = 4).
