Table 1.
EXiLE in RS-ATL8 cells sensitized with egg-allergy patients' sera
| EXiLE (fold) by EWP (ng/ml) | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient | Age, Sex | OFC* test | totIgE (IU/ml) | CAP (UA/ml) | CAP class | CAP* test† | 1 | 10 | 100 | 1000 | Max EXiLE (fold) | EXiLE score‡ | EXiLE* test§ | Anti-IgE EXiLE (fold) |
| #79 | 6, M | + | 1922 | 76.8 | 5 | + | 2.3 | 3.4 | 4.5 | 3.5 | 4.5 | 4 | + | 16.7 |
| #77 | 13, M | + | 1273 | 68.6 | 5 | + | 4.1 | 8.8 | 9.5 | 7.5 | 9.5 | 4 | + | 17.9 |
| #78 | 9,F | + | 844 | 48.3 | 4 | + | 4.8 | 8.3 | 8.4 | 8.6 | 8.6 | 4 | + | 17.6 |
| #80 | 6,M | + | 381 | 35.1 | 4 | + | 1.5 | 3.0 | 3.9 | 5.0 | 5.0 | 3 | + | 12.0 |
| #74 | 9,M | + | 2663 | 29.9 | 4 | + | 3.9 | 3.3 | 4.5 | 4.8 | 4.8 | 4 | + | 20.9 |
| #90 | 14, F | + | 12323 | 25.6 | 4 | + | 1.5 | 2.5 | 2.8 | 2.8 | 2.8 | 3 | + | 16.7 |
| #84 | 5,M | + | 331 | 13.2 | 3 | + | 1.5 | 1.7 | 2.3 | 1.9 | 2.3 | 2 | + | 7.84 |
| #83 | 10, M | + | 772 | 8.98 | 3 | + | 1.3 | 1.7 | 2.5 | 3.2 | 3.2 | 2 | + | 17.7 |
| #85 | 8,M | + | 619 | 5.57 | 3 | + | 1.6 | 2.5 | 3.4 | 3.1 | 3.4 | 3 | + | 24.2 |
| #81 | 10, F | + | 795 | 4.54 | 3 | + | 1.5 | 2.2 | 1.8 | 2.7 | 2.7 | 3 | + | 19.5 |
| #76 | 6,M | + | 509 | 3.91 | 3 | + | 1.4 | 1.7 | 2.0 | 2.2 | 2.2 | 2 | + | 23.0 |
| #94 | 9,F | + | 653 | 2.99 | 2 | + | 0.8 | 1.3 | 1.3 | 1.5 | 1.5 | 0 | − | 11.8 |
| #73 | 9,F | − | 7339 | 33.4 | 4 | + | 1.8 | 2.2 | 2.2 | 1.9 | 2.2 | 3 | + | 26.9 |
| #67 | 13, M | − | 22600 | 4.46 | 3 | + | 1.0 | 1.2 | 1.4 | 1.6 | 1.6 | 0 | − | 14.2 |
| #82 | 10, M | − | 1470 | 3.45 | 2 | + | 1.2 | 1.2 | 1.5 | 1.4 | 1.5 | 0 | − | 16.7 |
| #95 | 5,F | − | 578 | 2.03 | 2 | + | 1.1 | 1.2 | 1.3 | 1.5 | 1.5 | 0 | − | 10.0 |
| #75 | 6,M | − | 1006 | 1.17 | 2 | + | 0.8 | 0.9 | 0.9 | 1.0 | 1.0 | 0 | − | 25.0 |
| #100 | 9,M | − | 5356 | 0.89 | 2 | + | 0.9 | 1.3 | 1.5 | 1.4 | 1.5 | 0 | − | 23.0 |
| #99 | 13, M | − | 475 | <0.34 | 0 | − | 1.1 | 1.1 | 1.3 | 1.5 | 1.5 | 0 | − | 7.13 |
RS-ATL8 cells were sensitized with l : 100-diluted egg-allergy patients' sera overnight, and stimulated with the indicated concentrations of EWP or 1 μg/ml anti-IgE for 3 h.
Correlation between OFC and CAP tests was P = 0.3684, and that between OFC and EXiLE tests was P = 0.001687 (Fisher's exact test).
CAP test was considered positive if the class was ≥2 (i.e. ≥0.70 UA/ml)
EXiLE score varies from 0 to 4. Score 1, 2, 3, and 4 means its EXiLE exceeds 2.0 at EWP concentrations of 1000, 100, 10, and 1 ng/ml, respectively. Score 0 means negative.
EXiLE test was judged to be positive if Max EXiLE was more than the cut-off level (2.0).
Values greater than cut-off levels are represented in Bold. EWP, Egg white proteins; EXiLE, IgE crosslinking–induced luciferase expression; OFC, oral food challenge.