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. 2010 Oct;65(10):1256–1265. doi: 10.1111/j.1398-9995.2010.02359.x

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Copyright © 2010 John Wiley & Sons A/S

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Protein components of EVs secreted in response to LPS are key mediators in the development of mixed Th1 and Th17 cell responses to inhaled allergens. (A) Study protocol for in vivo experiments to evaluate adaptive immune responses (n = 5 each group). (B) Bronchoalveolar lavage (BAL) cellularity. *P < 0.05. (C) Intracellular staining for Th1 (IFN-γ) and Th17 (IL-17) cytokines: FACS data were derived from CD3⁺ gated lung cells. (D) The levels of Th1 (IFN-γ), Th17 (IL-17), and Th2 (IL-4) cytokines in supernatants 6 h after lung CD4+ T cells were incubated with PBS or anti-CD3 plus anti-CD28 antibodies. *P < 0.05.