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. 2011 Jan 5;32(4):537–544. doi: 10.1093/carcin/bgq285

Fig. 4.

Fig. 4.

Treatment of A431 cells with EGCG or 5-aza-dc for 3 or 6 days inhibits DNMT activity and decreases the levels of mRNA and protein expressions of DNMT1, DNMT3a and DNMT3b. (A) total DNMT activity in nuclear extracts was determined using the DNMT Activity Assay Kit. Data are presented in terms of percentage versus the results using non-EGCG-treated controls, which was assigned a value of 100%, and as means ± standard deviations; n = 3. (B) Quantitative real-time PCR analysis of mRNA levels of DNMT1, DNMT3a and DNMT3b in cells. The results are presented as the expression of the individual mRNA with normalization to β-actin and as mean values ± standard deviations, n = 3. Significant difference versus non-EGCG-treated controls; ***P < 0.05, **P < 0.01, *P < 0.001. (C) The levels of DNMT1, DNMT3a and DNMT3b in cell lysates were determined using western blot analysis after treating the cells with EGCG for 6 days. The treatment groups of 5-aza-dc and TSA were used as controls.