Fig. 3.

Function assays for SNPs in the promoter of TNFSF8. (A) EMSA with biotin-labeled oligo contained the wild- type (W) allele, variant (M) allele for four putative functional SNPs and nuclear extracts from H1299 cells. (B) Schematic of the position of rs2075533 SNP relative to the transcription start site. (C) Luciferase expression of constructs in H1299, HeLa and HCT116 cell lines. Values represent fold change of luciferase activity relative to the wild-type constructs as one. Each bar represents the mean of triplicate transfected plates plus standard deviation. *P < 0.001, compared with the wild-type construct.