Fig. 5.

Effect of oral administration of 2HF on tumor regression of RCC in nude mice: For 786-O RCC, mice were divided into four groups treated with corn oil (i.e. vehicle) and 2HF 0.0025, 0.005 and 0.01% (wt/wt) (equivalent to 25, 50 and 100 mg/kg body wt, respectively). For Caki-2 RCC, mice were divided into two groups treated with corn oil, and 2HF 0.01% (wt/wt) (equivalent to 100 mg/kg body wt). Experimental details are given in the Methods section. Animals were examined daily for signs of tumor growth and body weights were recorded (panels A). Photographs of animals were taken at day 1, day 10, day 20, day 40 and day 60 after subcutaneous injection are shown for all groups (data not shown). Weights and photographs of tumors were also taken at day 60 (panels B). Tumors were measured in two dimensions using calipers (panels C).