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. Author manuscript; available in PMC: 2011 Mar 30.
Published in final edited form as: J Biol Chem. 2000 Sep 29;275(39):30740–30745. doi: 10.1074/jbc.M001702200

Fig. 4. Ras-GTP is unaltered in fibroblasts from Nf1 null mice.

Fig. 4

Fibroblasts from wild type (A–C) or Nf1−/− mice (D and E) were grown in DMEM in the absence of serum for 24 h and then treated with 20 ng/ml insulin for 30 min (B and E) or 1 h (C and F) and examined by Ras-GTP immunocytochemistry. Fibroblasts from these same Nf1−/− embryos demonstrated increased proliferation and collagen deposition (not shown), characteristic features of mouse fibroblasts lacking neurofibromin. Note that Ras-GTP levels were similar in Nf1−/− and wild type fibroblasts and that Ras-GTP levels decreased over a similar time course in both cell populations.