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. 2001 Mar 6;98(6):3398–3403. doi: 10.1073/pnas.041609498

Figure 1.

Figure 1

Immunization with pMOG or passive transfer of anti-MBP T cells does not protect mouse RGCs from glutamate toxicity. (A) C57BL/6J mice were immunized with pMOG 14 days before their RGCs were exposed directly to glutamate toxicity by intravitreous injection of l-glutamate (200 nmol). Four days later the RGCs were retrogradely labeled with FluoroGold, followed after 3 days by retinal excision and counting (see Materials and Methods). RGC survival is expressed as the mean ± SEM per square millimeter. No significant differences in RGC survival after glutamate injection were observed between the group treated with pMOG in CFA (n = 8) and the control group treated with PBS in CFA (n = 7). (B) Glutamate was injected intravitreally into Lewis rats. Four days later, the RGCs were labeled by application of the dye 4-(4-(didecylamino)styryl)-N-methylpyridinium iodide, followed after 5 days by retinal excision and counting. Note that retinal survival in the T-cell-treated group did not differ significantly from that in the control group (no. of RGCs per square millimeter, mean ± SEM; n = 6 in each group).