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. 2010 Jul 21;86(7):757–768. doi: 10.2183/pjab.86.757

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© 2010 The Japan Academy

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Expression of chimeric OMTs with N- and C-terminals derived from different OMTs. (A) The crude E. coli lysate (10 µg protein) expressing the chimeric OMTs was separated by SDS-PAGE and stained with Coomassie Brilliant Blue R250 (CBB). The molecular mass of the chimeric OMTs is ca. 40 kDa (indicated by arrow). (B) Immunoblot analysis with anti-4′-OMT polyclonal antibodies. (C) Immunoblot analysis with anti-SMT polyclonal antibodies. M, molecular mass markers; lane 1, pET-4′OMT; lane 2, pET-64′OMT; lane 3, pET-4′6OMT; lane 4, pET-S4′OMT; lane 5, pET-4′SOMT; lane 6, pET-S6OMT; lane 7, pET-6SOMT; lane 8, pET-SMT; lane 9, pET-21d (vector control).