Figure 4.

DiI-oxLDL (10 µg/mL) binding in LOX-1-CHO cells in the presence of various isomers of trimer procyanidins. Fluorescent images of DiI-oxLDL (red) binding to LOX-1-CHO cells in the absence or presence of procyanidin C1 (300 ng/mL) or in the presence of anti-LOX-1 antibody (A). Nuclei were stained by DAPI (blue). DiI-oxLDL binding in LOX-1-CHO cells in the presence of various concentrations of trimer procyanidin isomers (B). Chemical structures of the trimer procyanidin isomers used in the experiments are shown. Difference in the hydroxyl groups indicated by red jagged lines causes stereoisomers of catechin and epicatechin.