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Co-immunoprecipitation assays with alanine substitution mutants. The dimerization ability of Flag-tagged wt or mutant LANADBDs with HA-tagged wt LANADBD was tested. Dimerization activity for each mutant was normalized based on the expression level of Flag-tagged wt or mutant LANADBD proteins. L, Cell lysate, IP; immunoprecipitated samples; Wt (N), HA-tagged wt only as a negative control; Wt (P), Flag-tagged and HA-tagged wt as a positive control.
