Table 1.
Top-Down Confirmed Protein Identifications and Their Associated Parameters
| Protein ID | Predicted Mass (Da)* (mono/avg) | Residues | Localization (from MALDI Imaging Data) |
Solubility (from MALDI Profiling Data) |
||
|---|---|---|---|---|---|---|
| 21-day ICR/f | 100-day ICR/f | 21-day ICR/f | 100-day ICR/f | |||
| Crystallin, alpha A† | 5,053.50 / 5,056.84 | 1–42 | Mostly nuclear | Mostly nuclear | Mostly insoluble | Insoluble |
| Crystallin, alpha A† | 6,409.19 / 6,413.39 | 1–53 | Nuclear | Nuclear | Insoluble | Insoluble |
| Crystallin, alpha A† | 6,565.29 / 6,569.58 | 1–54 | Nuclear | Cortical ring | Insoluble | Insoluble |
| Crystallin, alpha A† | 9,284.76 / 9,290.70 | 1–78 | Nuclear | Nuclear | Insoluble | Insoluble |
| Crystallin, alpha A† | 9,421.80 / 9,427.84 | 1–79 | Nuclear | Mostly nuclear | Insoluble | Insoluble |
| Crystallin, alpha A† | 10,110.11 / 10,116.58 | 1–85 | Mostly nuclear | Nuclear | Insoluble | Insoluble |
| Crystallin, alpha A† | 11,041.61 / 11,048.68 | 1–93 | Nuclear | Nuclear | Insoluble | Insoluble |
| Crystallin, alpha A† | 11,842.04 / 11,849.60 | 1–100 | Nuclear | Mostly nuclear | Insoluble | Insoluble |
| Crystallin, alpha A† | 11,956.08 / 11,963.70 | 1–101 | Nuclear | Mostly nuclear | Sol/insol | Insoluble |
| Crystallin, alpha A† | 17,562.77 / 17,573.90 | 1–15§ | Nuclear ring | Nuclear ring (dispersed) | Mostly insoluble | Mostly insoluble |
| Crystallin, alpha A† | 18,043.96 / 18,055.37 | 1–156§ | Nuclear ring | Nuclear ring (dispersed) | Sol/insol | Mostly insoluble |
| Crystallin, alpha A† | 18,200.06 / 18,211.56 | 1–157§ | Nuclear ring | Nuclear ring (dispersed) | Sol/insol | Sol/insol |
| Crystallin, alpha A† | 18,823.44 / 18,835.32 | 1–163§ | Nuclear ring | Cortical | Sol/insol | Sol/insol |
| Crystallin, alpha A† | 19,393.70 / 19,405.92 | 1–168§ | Nuclear ring | Cortical | soluble | Soluble |
| Crystallin, alpha A† | 19,822.89 / 19,835.35 | 1–173§ | Cortical | Outermost cortical | soluble | Soluble |
| Alpha-crystallin A chain‡ | 9,384.59 / 9,390.84 | 1–79 | Nuclear ring | Cortical/Nuclear | Sol/insol | Mostly insoluble |
| Alpha-crystallin A chain‡ | 9,837.83 / 9,844.35 | 1–83 | Nuclear ring | Cortical/Nuclear | N/A | Mostly insoluble |
| Alpha-crystallin A chain‡ | 22,476.15 / 22,490.49 | 1–196 | Cortical | Outermost cortical | Soluble | Soluble |
The identities are correlated with the localization data taken from the MSI experiments as well as the intrinsic solubilities from the MALDI-TOF profiling experiments.
*
N-terminal acetylation included (+42.01 Da).
†
UniProtKB/TrEMBL A0JN13 (A0JN13_RAT).
‡
UniProtKB/Swiss-Prot P24623 (CRYAA_RAT).
§
Previously identified in ICR/f rat.17