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. 2010 Oct;51(10):5190–5197. doi: 10.1167/iovs.10-5257

Figure 3.

Figure 3.

Knockdown of NBCe1 expression. (A) Representative immunofluorescence images (blue, nuclear DAPI; green, NBCe1) of corneal endothelial peelings from the right (OD, Lenti-scrambled) and left (OS, Lenti-NBCe1-shRNA) corneas 4 weeks after injection. Scale bar, 100 μm. (B, left) Representative real-time PCR products. Right: summary results from eight rabbits and only those showing >25% knockdown. (C) Representative Western blot analysis 14 and 28 days after injection. (D) Summary of Western blot analysis (n = 34) and quantitative PCR (n = 8) data from all rabbits analyzed (n = 42) and subdivided into those coexpressing GFP (n = 24) and without GFP (n = 18). (E) Rabbits that had >25% knockdown of NBCe1 (n = 30) and subdivided at 2 weeks (n = 19) and 4 weeks (n = 11) after injection.