Figure 4.

CTGF did not colocalize at focal adhesions with FAK, vinculin, or integrin α₅β₁ or α_vβ₃. HCFs growing on CL-coated coverslips were scrape-wounded and incubated with TGF-β for 10 hours and then fixed, permeabilized, and immunodetected for CTGF (red) and FAK, vinculin, and integrin α₅β₁ or α_vβ₃ (green). In the cells migrating into the wound (asterisk), CTGF was detected in intracellular vesicles and the Golgi (arrows). Focal adhesions enriched in FAK, vinculin, α₅β₁, or α_vβ₃ (arrowheads) did not colocalize with intracellular or extracellular CTGF (A–D). Scale bars, 10 μm.