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Immunodepletion of hAH. (A) Eighty-five hAH samples were divided into three groups and immunodepleted of albumin, transferrin, antitrypsin, haploglobin, IgG, and IgA. Groups were separated on 10% to 14.5% SDS-PAGE gradient gels. PD, predepletion; D, immunodepleted (flow-thru); E, eluted from column. (B) Thirty-three gel slices were isolated from each group, independently trypsinized, and processed for nano-LC-ESI-MS/MS. G1, group 1; G2, group 2: G3, group 3.
