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. 2010 Oct;51(10):5328–5335. doi: 10.1167/iovs.09-5015

Figure 2.

Figure 2.

Misexpression of β-catenin or Otx2 is not sufficient to induce Mitf in the presumptive chick retina. (A–F) A bicistronic expression vector containing CA-β-catenin and EGFP (βcatEGFP) was electroporated into E1.5 chick optic vesicles that were examined 2 days later. (A, D; same image) EGFP detected with GFP antibody marks transfected regions. (B, E) Mitf and Otx2 proteins were normally expressed in the RPE (brackets), but no ectopic expression was observed in the neural retina. (C, F) Merged files. (G–I) Electroporation of Otx2 (Otx2HA) into presumptive chick retina at E1.5 followed by examination for gene expression at E3.5. (G) Ectopic Otx2 expression, detected by HA immunolabeling, was observed in several cells of the retina. (H) Mitf was not detected in the transfected cells. (I) Merged image.