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. 2010 Oct;51(10):5328–5335. doi: 10.1167/iovs.09-5015

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Figure 4. — Misexpression of β-catenin and Otx2 induced Mitf expression. (A–D) E3.5 chicken retinas transfected with Otx2 (Otx2HA) and β-catenin (βcatEGFP) were stained with HA (A; red), GFP (B; green), and Mitf antibodies (D; red) and counterstained with DAPI (blue). (C) Merged image (yellow nuclei): coexpression of β-catenin and Otx2. (A–D) arrows: Co-transfected cells with strong HA signals; arrowheads: weakly HA-transfected cells. (D) Co-transfected cells showed induction of ectopic Mitf expression in the retina. (E) Ectopically Mitf-expressing cells were counted and categorized according to co-localization with EGFP (βcatEGFP) or HA (Otx2HA). Expression vectors containing only EGFP and HA were used as the control. ANOVA revealed statistical significance between the experimental and the control groups (P < 0.0001). (F) The percentage of cells with ectopic Mitf expression was higher in co-transfected cells with strong HA staining.