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. 2001 Feb 27;98(6):3416–3421. doi: 10.1073/pnas.051378298

Figure 5.

Figure 5

Comparison of caspase-3 activation by DCC-IC versus cytochrome c. A dose-dependence analysis of DCC-induced caspase-3 activation was performed. In a similar in vitro assay as in Fig. 4, different quantities of DCC-IC or cytochrome c in the presence or not of 1 mM dATP were added. In the case of DCC-IC, the concentration was estimated based on comparison with purified protein (L.G. and P.M., unpublished data). Purified cytochrome c was used as attempts to activate caspase-3 in vitro with in vitro-translated cytochrome c were unsuccessful, probably as a result of the absence of the cytochrome c-associated heme (C.F. and P.M., unpublished results). The p20 cleavage fragment of caspase-3 was then detected by immunoblot and quantified by densitometry. A graph of the level of caspase-3 activation is presented.