TABLE 2.

Oligonucleotides and plasmids used in this study

Primer or plasmid	Sequencea	Use in this study	Source or reference
qnr cloning
Pre-QnrA1	5′-CGGGATCCCGCGGCAGTTAAAATTGGGGCT-3′	Cloning of qnrA1	This study
Post-QnrA1	5′-CGGGATCCCGACGCCGAGTCCCGACCAGACTGC-3′	Cloning of qnrA1	This study
Pre-QnrB1	5′-CGGGATCCCGCTTGGTCGCCCTGGCCAACC-3′	Cloning of qnrB1	This study
Post-QnrB1	5′-CGGGATCCCGGCAAACCAGCTTACAGCAGGC-3′	Cloning of qnrB1	This study
Pre-QnrS1	5′-CGGGATCCCGCCACTTAAAACAGGTAAATTG-3′	Cloning of qnrS1	This study
Post-QnrS1	5′-CGGGATCCCGTACATGGTTGTCCCTATGTC-3′	Cloning of qnrS1	This study
Gene replacement
gyrAS83L-Fw	5′-CCATGGTGACCTGGCGGTCTATG-3′	Mutagenesis of gyrA	This study
gyrAS83L-Rv	5′-CATAGACCGCCAGGTCACCATGG-3′	Mutagenesis of gyrA	This study
parCS80R-Fw	5′-CCGCACGGCGATCGCGCCTGTTATGAAGC-3′	Mutagenesis of parC	This study
parCS80R-Rv	5′-GCTTCATAACAGGCGCGATCGCCGTGCGG-3′	Mutagenesis of parC	This study
Pre-gyrAS83	5′-CGGGATCCCGAGCGATCTCTTCGTGGTCTACG-3′	Partial gyrA amplification	This study
Post-gyrAS83	5′-CGGGATCCCGCCTGATACGGAATTTCGTGGAC-3′	Partial gyrA amplification	This study
Pre-parCS80	5′-CGGGATCCCGGACCGCGATAGCGTTGTCTTCCG-3′	Partial parC amplification	This study
Post-parCS80	5′-CGGGATCCCGCAGATCGGTGGTAGCGAAGAGGTG-3′	Partial parC amplification	This study
QRDRb sequencing
gyrA-1	5′-AAATCTGCCCGTGTCGTTGGT-3′	Sequencing	17
gyrA-2	5′-GCCATACCTACGGCGATACC-3′	Sequencing	17
parC-A	5′-CTGAATGCCAGCGCCAAATT-3′	Sequencing	17
parC-B	5′-GCGAACGATTTCGGATCGTC-3′	Sequencing	17
Plasmids
pBK-CMV	Cloning vector
pST76C	Gene replacement/suicide vector
pUC19RP12	Gene replacement/resolution vector

^aUnderlined nucleotides correspond to the BamHI site used for cloning.

^bQRDR, quinolone resistance-determining region.