Figure 3.
The levels of NHE1 phosphorylation in anti-IgM-treated B104 cells. B104 cells (5 × 105/ml), pretreated or not with 200 ng/ml CsA for 30 min, were stimulated with 20 μg/ml anti-IgM for the indicated times. Immunoprecipitates isolated from cell lysates with either normal rabbit serum (NRS) or rabbit anti-NHE1 serum were subjected to a 7.5% SDS/PAGE, transferred to poly(vinylidene difluoride), and immunoblotted with either rabbit anti-phosphoserine antibody or rabbit anti-NHE1 serum. The blots were developed by the ECL method. This is a representative experiment of three experiments performed.