TABLE 2.
Primers used in PCR and sequencing studies for antimicrobial resistance genes
| Target gene(s) | Primer name | Sequence (5′ to 3′) | Position in Fig. 1 |
|---|---|---|---|
| blaTEM | TEM-F | TCCGCTCATGAGACAATAACC | |
| cluster | TEM-R | ACGCTCAGTGGAACGAAAAC | |
| blaSHV | SHV-F | CGCCGGGTTATTCTTATTTG | |
| cluster | SHV-R | CCACGTTTATGGCGTTACCT | |
| blaVEB | VEB-F | AAAATGCCAGAATAGGAGTAGCA | |
| cluster | VEB-R | TCCACGTTATTTTTGCAATGTC | |
| blaGES | GES-F | CGCTTCATTCACGCACTATT | |
| cluster | GES-R | GTCCGTGCTCAGGATGAGTT | |
| blaCTX-M-1 | CTX-M-1F | CCGTCACGCTGTTGTTAGG | |
| cluster | CTX-M-1R | ACGGCTTTCTGCCTTAGGTT | |
| blaCTX-M-9 | CTX-M9-F | CAAAGAGAGTGCAACGGATG | |
| cluster | CTX-M9-R | CCTTCGGCGATGATTCTC | |
| blaPER-1 | PER-F | CCTGACGATCTGGAACCTTT | 1 |
| cluster | PER-R | TGGTCCTGTGGTGGTTTC | 2 |
| ISPa12 gene | ISPa12-F | AAGCCCTGTTTTCAGAGCAA | 3 |
| ISPa12-R | AATCAACGTTTCGGCTATCG | 4 | |
| ISPa12-mF | GCCGATGCAGGTTATTTTTC | 5 | |
| ISPa12-wR | TCATGATTCATATGTGATTTCCAA | 6 | |
| ISPa13 gene | ISPa13-F | TTTTCAGCAGCAGAGCTTGA | 7 |
| ISPa13-R | CGTTGATTAGCCAGCGTTTT | 8 | |
| ISPa13-mF | TGATAAAGAGGCGGGTGAAG | 9 | |
| ISPa13-wR | TTTACGCCTCATAGGTATGATCTTTAG | 10 | |
| gst gene | GST-F | CCCCTTTTGTTCGTCGTTTA | 11 |
| GST-R | AAGGAGTCTGTGCAGGCATT | 12 |