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pL102 presentation and shortening description. pL102 is composed of a 13.5-kb DNA fragment (▪) inserted in the pUC19 vector (□).The mclC structural gene, the mclI immunity gene, and the mclA and mclB export genes constitute the MccL genetic system. The restriction sites used for the insert shortening were XbaI/SacI on one side and HindIII/PauI on the other side. The HindIII-PauI fragment was deleted. The dotted arrows indicate the start points and the direction of the shortening.
