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Depolarization of the bacterial cytoplasmic membrane by MccL. The fluorescence of the dye DiSC3(5) within E. coli MC4100 bacterial suspension was monitored at 37°C with a spectrophotometer (excitation wavelength, 643 ± 2.5 nm; emission wavelength, 666 ± 2.5 nm). (A) The arrow indicates the addition of 6 μM MccL (▴), 3.5 μM melittin (▪) (positive control), or MccL solvent (⧫) (negative control). (B) Each arrow indicates the addition of 2 μM MccL.
