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. 2010 Dec 13;55(3):1173–1176. doi: 10.1128/AAC.00817-10

TABLE 1.

Oligonucleotide primers used in this study

Determinant and location Target gene Primer sequence (5′ to 3′) Expected amplicon size (kb)
fusB in SaRIfusB SAS1815 TAAAAAAATCTACTCAAAAC 2.7
fusB TAAATGATAAAGAAACCGTC
fusB in pUB101 or pUB102 blaZ TAGATACTAAAAGTGGTAAGG 3.4 (pUB101), 2.6 (pUB102)
fusB CTATAATGATATTAATGAGATTTTTGG
fusC in SCC476 SAS0040 AGGTGGATTGATGGGAGTTAAG 1.3
fusC ATTATTTATATCATCTAGGTTCTG
fusC in SCC476 SAS0045 AGTTCTATACTGAAGGTTATGG 2.6
fusC TTAAAGAAAAAGATATTGATATCTCGG
fusC in SCCmecN1 Upstream of fusC within SCCmecN1 CTAATATGTTGGCGCTGATAT 10.1 or 3.7a
fusC ACAAACGATATGAATTCCCA
fusC (SCCmecN1)b ccrAB4-1CHE482-ccrAB4-2CHE482 CAAATGATTGAAACAGAGGT 0.8
ccrAB4-1CHE482-ccrAB4-2CHE482 CACGTTTTCTACAATAACGT
a

Depending on the SCCmecN1 variant (6).

b

The primers do not detect fusC directly but probe for the fusC-associated element SCCmecN1 (6).