TABLE 2.
Oligonucleotides used for gene inactivation, gene cloning, and RT-qPCR
| Oligonucleotide | Sequence (5′→3′) |
|---|---|
| Oligonucleotides for gene inactivation | |
| mexZ | |
| PCRimexZC1 | CGTAGCGCTTGAGCTTGTC |
| PCRimexZC2 | GCCCTCACACTGAACGTCCTCACAAGG |
| PCRimexZC3 | TTCAGTGTGAGGGCGTCAATCGTCC |
| PCRimexZC4 | GGTTCGTAGATCGGCATCTC |
| PA5471 | |
| PCRi5471C1 | CTCCTGCGCGAGGTCTAC |
| PCRi5471C2 | GGCGGTCGCGGACAAAGGCTTGATGTA |
| PCRi5471C3 | TGTCCGCGACCGCCAGCTGATCTACG |
| PCRi5471C4 | GATCGCCAGGCGTTTGTT |
| PA5470 | |
| PCRi5470C1 | CTGGAACTGCTCGACATCAA |
| PCRi5470C2 | GAAGCTGTCCTCGATTACCTCGACCTC |
| PCRi5470C3 | TCGAGGACAGCTTCACTGGCTCTTCG |
| PCRi5470C4 | CCGACCTCTTCCACCTGTT |
| parS | |
| PCRiparSC1 | AGGAGGACGATCTCGATCAC |
| PCRiparSC2 | AGATCTTGGTCGACCACGAAGATCG |
| PCRiparSC3 | GTCGACCAAGATCTTCGAACCCTTTTCG |
| PCRiparSC4 | GCGTTGATCCTGTGCTGTC |
| parRS | |
| PCRiParRSC1 | GGATTCAGCTTCTGCTCGAC |
| PCRiParRSC2 | AGATCTTCATAGGGTTTCATCGGTCG |
| PCRiParRSC3 | CCCTATGAAGATCTTCGAACCCTTTTCG |
| PCRiParRSC4 | GCGTTGATCCTGTGCTGTC |
| pmrAB | |
| PCRiPmrABC1 | TGTCGAACTGACCCAGCTAC |
| PCRiPmrABC2 | CTTCCAGGTCACCCATTCCACGGTATC |
| PCRiPmrABC3 | GGGTGACCTGGAAGTGCAGGTGTTCCT |
| PCRiPmrABC4 | GTGCTGAGCTCCTCGATCTT |
| Oligonucleotides for complementation of parRS | |
| CloparRSC1 | GAGGGAAAAGCAGAAGTCACC |
| CloparRSC2 | CGAGGTGTCCCATGCTAGG |
| Oligonucleotides for RT-qPCR | |
| uvrD1 | GCAGCCTCGCCCTACAGCA |
| uvrD2 | GGATCTGGAAGTTCTGCTCAGC |
| mexY1A | GCAGCCTCGCCCTACAGCA |
| mexY1B | GGATCTGGAAGTTCTGCTCAGC |
| mexZRTA1 | TTACCTCCTCCAGCGGC |
| mexZRTA2 | GTGAGGCGGGCGTTGTG |
| PA5470C1 | CCAAAGAGGAATCCCAGAAA |
| PA5470C2 | CAGGCAGACCTCGATCTTGT |
| PA5471C1 | TCGAGGTAATCGAGGAGGTG |
| PA5471C2 | AGGGTCTGCAAACGGATCTC |
| oprDRTC1 | CATCTACCGCACAAACGATG |
| oprDRTC2 | ACAGAGTTGGCGAGGAAAATC |
| parRSTC1 | CGAACTGGAGGAAATGGTCT |
| parRSTC2 | ATGCGGATCTGTTCGACCT |
| pmrARTC1 | GATACCGTGGAATGGGTGAC |
| pmrARTC2 | GGCTTGGTCAGGTAGTCGTC |
| pmrBRTC1 | ACGAACTCAACCTCCTGCTG |
| pmrBRTC2 | ATCTGCTCCATCAAGGTGCT |
| PA1559RTC1 | GCAGCAACTGGTGGACTACA |
| PA1559RTC2 | CATGCGGAAGACCAGAAGAT |
| PA1797RTC1 | GGACCCTTTGCAGATGACTC |
| PA1797RTC2 | CGGAGTGTTTCCTGAGAAGC |
| PA2358RTC1 | GTACTGTTCGCCGGAACAAC |
| PA2358RTC2 | CCTGGAGCAGGAATATCTGG |
| PA2655RTC1 | GTGCTGGTGTTCCTGTTGG |
| PA2655RTC2 | CGTAGGTCCCCCAGATCG |
| PA3554RTC1 | GTGGCTCGAATACCATGTGA |
| PA3554RTC2 | TGCCGTATTTCACGCAGTAG |
| PA4773RTC1 | CAGTGGATCGAGGAAAGCAT |
| PA4773RTC2 | GTACTCCGGCCAGGTATGG |
| PA4774RTC1 | CGCCGAACCACTTCTATTTC |
| PA4774RTC2 | TCGTGGTACAGCGACTCATC |