TABLE 3.
Drug susceptibilities of various mutants derived from strains PAO1Bes and CMC
| Strain | Genotype | MIC (μg/ml)a |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| GEN | AMK | TOB | APR | FEP | CIP | IMP | MEM | CST | ||
| PAO1Bes | Wild type | 2 | 4 | 1 | 16 | 4 | 0.25 | 2 | 1 | 1 |
| CMC | Wild type | 2 | 4 | 1 | 16 | 4 | 0.25 | 2 | 1 | 1 |
| PAOW1 | PAO1Bes agrW1 mutant | 16 | 32 | 4 | 64 | 16 | 1 | 2 | 1 | 1 |
| PAOW2 | PAO1Bes agrW2 mutant | 8 | 32 | 2 | 64 | 8 | 1 | 16 | 4 | 2 |
| CMCW2 | CMC agrW2 mutant | 4 | 8 | 2 | 32 | 8 | 0.5 | 16 | 4 | 2 |
| CMZ091 | PΑΟ1 ΔmexZ | 8 | 16 | 2 | 32 | 8 | 1 | 2 | 1 | 1 |
| CM090 | PΑΟ1 ΔPA5470 | 2 | 4 | 1 | 16 | 4 | 0.25 | 2 | 1 | 1 |
| CM093 | PΑΟ1 ΔPA5471 | ≤0.12 | 2 | 0.5 | 4 | 4 | 0.12 | 2 | 1 | 1 |
| CM095 | PΑΟ1 ΔparS | 2 | 4 | 1 | 16 | 4 | 0.25 | 2 | 1 | 0.5 |
| CM096 | PAO1 ΔparRS | 2 | 4 | 1 | 16 | 4 | 0.25 | 2 | 1 | 0.5 |
| CM099 | PΑΟ1 ΔpmrAB | 2 | 4 | 1 | 16 | 4 | 0.25 | 2 | 1 | 0.5 |
| CMZ089 | PΑΟW2 ΔmexZ | 8 | 32 | 2 | 64 | 8 | 1 | 16 | 4 | 2 |
| CM092 | PΑΟW2 ΔPA5470 | 8 | 32 | 2 | 64 | 8 | 1 | 16 | 4 | 2 |
| CM094 | PΑΟW2 ΔPA5471 | 8 | 32 | 2 | 64 | 8 | 1 | 16 | 4 | 2 |
| CM097 | PΑΟW2 ΔparS | 2 | 4 | 1 | 16 | 4 | 0.25 | 2 | 1 | 0.5 |
| CM098 | PΑΟW2 ΔparRS | 2 | 4 | 1 | 16 | 4 | 0.25 | 2 | 1 | 0.5 |
| CM100 | PΑΟW2 ΔpmrAB | 8 | 32 | 2 | 64 | 8 | 1 | 16 | 4 | 1 |
| CM106 | CM096 with parRS from PAOW2 | 4 | 8 | 1 | 32 | 8 | 0.5 | 16 | 4 | 1 |
| CM107 | CM096 with parRS from CMCW2 | 4 | 8 | 1 | 32 | 8 | 0.5 | 16 | 2 | 1 |
a
MICs in bold (or underlined) indicate that the bacteria were at least 2-fold more (or less) resistant than the reference strain PAO1Bes. The data are representative of four distinct experiments. Abbreviations: GEN, gentamicin; AMK, amikacin; TOB, tobramycin; APR, apramycin; FEP, cefepime; CIP, ciprofloxacin; IMP, imipenem; MEM, meropenem; and CST, colistin.