Evaluation of prophylactic effect of PC-SA-associated PM. Normal mice were treated once with PC-SA, PM, and PC-SA-PM at doses described in the legend to Fig. 6. Animals were infected with parasites 10 days after treatment and sacrificed after 12 weeks of infection. Liver (A) and spleen (B) parasite burden. (C) IFN-γ and IL-12 were induced, and IL-10 and TGF-β were inhibited in mice prophylactically treated with PC-SA-PM. Spleen cell culture supernatants of 12-week-infected BALB/c mice after indicated treatments were analyzed for LAg-specific cytokine secretion through ELISA. Data represent the mean ± SE for five animals per group. Data were tested by ANOVA. Differences between means were assessed for statistical significance by Tukey's test (**, P < 0.01; ***, P < 0.001).