TABLE 1.
Effect of N348I mutation on NNRTI drug susceptibility of molecular clones and clinical isolates of HIV-1a
| Subtype | NVP |
EFV |
ETV |
|||
|---|---|---|---|---|---|---|
| Mean EC50 ± SEM | Fold change (P value) | Mean EC50 ± SEM | Fold change (P value) | Mean EC50 ± SEM | Fold change (P value) | |
| Molecular clones | ||||||
| p8.9 B (WT)b | 55.64 ± 7.35 | 2.73 ± 1.39 | 1.31 ± 0.16 | |||
| p8.9 B (N348I) | 235.36 ± 19.83 | 4.23 (<0.02) | 7.69 ± 3.75 | 2.81 (NS) | 2.75 ± 0.25 | 2.10 (<0.02) |
| MJ4 C (WT) | 65.85 ± 4.2 | 2.82 ± 1.23 | 1.20 ± 0.18 | |||
| MJ4 C (N348I) | 287.33 ± 13.05 | 4.36 (<0.05) | 7.65 ± 3.02 | 2.71 (NS) | 2.05 ± 0.31 | 1.71 (NS) |
| 94ug114.1.6 D (WT) | 153.8 ± 5.26 | 5.73 ± 2.63 | 2.47 ± 0.23 | |||
| 94 μg 114.1.6 D (N348I) | 584.9 ± 12.29 | 3.81 (<0.02) | 15.88 ± 6.72 | 2.77 (NS) | 7.26 ± 0.93 | 2.94 (<0.02) |
| Clinical isolates | ||||||
| A 1 (WT) | 157.56 ± 17.69 | 5.76 ± 2.29 | 2.39 ± 0.31 | |||
| A 1 (N348I) | 849.24 ± 41.56 | 5.39 (<0.02) | 20.03 ± 7.38 | 3.47 (NS) | 6.52 ± 0.89 | 2.72 (<0.02) |
| A2 (WT) | 146.67 ± 13.48 | 5.19 ± 0.75 | 2.77 ± 0.77 | |||
| A2 (N348I) | 838.95 ± 50.99 | 5.72 (<0.02) | 24.24 ± 2.21 | 4.67 (<0.05) | 8.22 ± 1.43 | 2.97 (NS) |
| B (WT) | 47.73 ± 4.61 | 2.57 ± 0.54 | 1.79 ± 0.42 | |||
| B (N348I) | 265.26 ± 21.56 | 5.56 (<0.02) | 9.99 ± 2.48 | 3.89 (<0.05) | 4.52 ± 0.73 | 2.53 (NS) |
| C (WT) | 69.96 ± 3.25 | 2.63 ± 0.33 | 2.26 ± 0.15 | |||
| C (N348I) | 281.94 ± 10.02 | 4.03 (<0.02) | 12.09 ± 4.91 | 4.60 (NS) | 4.26 ± 0.25 | 1.89 (<0.05) |
a
Data shown are mean drug EC50s (nM) for both mutant and wild-type viruses and mean fold differences between mutant and wild-type values. Standard errors of the mean from three independent experiments are shown. Statistical analyses using Student's t test compared the N348I mutant virus with the wild-type virus. NS, no statistically significant difference.
b
WT, wild type.