TABLE 2.
Effect of N348I mutation on RC of molecular clones and clinical isolates in different cell linesa
| Subtype | Mean % of wild-type RC ± SEM (fold decrease), P value |
||
|---|---|---|---|
| HEK 293T cells | Jurkat cells | SupT1 cells | |
| Molecular clones | |||
| p8.9 B | 55.57 ± 4.07 (1.80), <0.02 | 54.16 ± 5.5 (1.85), <0.02 | 62.91 ± 4.49 (1.59), <0.02 |
| MJ4 C | 34.65 ± 2.28 (2.89), <0.02 | 23.26 ± 1.57 (4.30), <0.02 | 26.35 ± 0.44 (3.80), <0.02 |
| 94ug114.1.6 D | 68.33 ± 0.94 (1.46), <0.02 | 67.55 ± 3.03 (1.48), <0.02 | 73.38 ± 2.66 (1.36), <0.02 |
| Clinical isolates | |||
| A1 | 42.28 ± 4.39 (2.37), <0.02 | 31.28 ± 4.28 (3.20), <0.02 | 37.80 ± 3.49 (2.65), <0.02 |
| A2 | 46.86 ± 1.31 (2.13), <0.02 | 40.25 ± 0.07 (2.48), <0.02 | 47.46 ± 0.84 (2.11), <0.02 |
| B | 73.20 ± 3.00 (1.37), <0.02 | 67.12 ± 4.32 (1.49), <0.02 | 81.92 ± 2.25 (1.22), <0.02 |
| C | 50.39 ± 4.16 (1.98), <0.02 | 41.87 ± 1.19 (2.39), <0.02 | 61.17 ± 3.91 (1.63), <0.02 |
a
Data are from mean RC values for mutant viruses which were calculated from the number of relative light units per nanogram of input p24 and expressed as a percentage of the wild-type value. Fold decreases in RC are shown in parentheses for comparisons of mutant and wild-type values. The standard error of the mean from two independent experiments is shown. Statistical analyses using Student's t test compared the N348I mutant virus with the wild-type virus.