TABLE 3.
Effect of N348I plus or minus M184V on RC of B and non-B clinical isolates or molecular clones in HEK 293T, Jurkat, and SupT1 cellsa
| Subtype and mutation(s) | Mean % of wild-type RC ± SEM (fold decrease) |
||
|---|---|---|---|
| HEK 293T cells | Jurkat cells | SupT1 cells | |
| A1 | |||
| N348I | 42.28 ± 4.39 (2.37) | 31.28 ± 4.28 (3.20) | 37.80 ± 3.49 (2.65) |
| N348I + M184V | 48.47 ± 5.51 (2.06) | 44.97 ± 5.34 (2.22) | 58.93 ± 4.44 (1.70) |
| M184V | 75.91 ± 3.59 (1.32) | 97.28 ± 3.24 (1.03) | 92.67 ± 4.32 (1.08) |
| p8.9 B | |||
| N348I | 55.57 ± 4.07 (1.80) | 54.16 ± 5.5 (1.85) | 62.91 ± 4.49 (1.59) |
| N348I + M184V | 65.19 ± 2.73 (1.53) | 72.15 ± 3.48 (1.39) | 80.78 ± 1.21 (1.24) |
| M184V | 83.49 ± 3.46 (1.20) | 98.93 ± 2.43 (1.01) | 98.94 ± 2.13 (1.01) |
| MJ4 C | |||
| N348I | 34.65 ± 2.28 (2.89) | 23.26 ± 1.57 (4.30) | 26.35 ± 0.44 (3.80) |
| N348I + M184V | 51.81 ± 5.97 (1.93) | 60.83 ± 4.27 (1.64) | 67.29 ± 5.14 (1.49) |
| M184V | 81.99 ± 3.02 (1.22) | 96.52 ± 3.58 (1.04) | 98.43 ± 1.13 (1.02) |
| 94ug114.1.6 D | |||
| N348I | 68.33 ± 0.94 (1.46) | 67.55 ± 3.03 (1.48) | 73.38 ± 2.66 (1.36) |
| N348I + M184V | 77.07 ± 4.49 (1.30) | 81.35 ± 3.65 (1.23) | 80.54 ± 3.46 (1.24) |
| M184V | 88.30 ± 0.02 (1.13) | 97.37 ± 1.26 (1.03) | 99.67 ± 0.32 (1.00) |
a
Data are mean RC values of mutant viruses which were calculated from the number of relative light units per nanogram of input p24 and expressed as a percentage of the wild-type value. Fold decreases in RC are shown in parentheses for comparisons of mutant and wild-type values. The standard error of the mean from two independent experiments is shown.