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. 2011 Jan 10;55(4):1391–1402. doi: 10.1128/AAC.01213-10

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Copyright © 2011, American Society for Microbiology

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FIG. 1. — Map of the orf1-yvqF-vraS-vraR operon and construction of mutants. (A) Wild-type operon structure, with the single autoregulatory promoter element indicated by an arrow (3) and the predicted transcriptional terminator (http://cmr.jcvi.org/) indicated by a stem-loop symbol. (B) Markerless deletion of orf1, leaving only the first 3 amino acids (aa) fused in frame to the last 8 aa. (C) YvqF was truncated by markerless insertion of an XhoI site and two in-frame stop codons after the 5th aa. (D) VraS mutant truncated by stop codons inserted after the 2nd aa. (E) VraR mutant truncated by stop codons inserted after the 2nd aa.