FIG. 5.
Induction of CWSS transcripts in vra operon mutants. (A) Transcriptional profiles of sas016 and the vra operon in BB270 and its four vra operon mutants. RNA was harvested from strains containing either the empty plasmid pAW17 or the transcomplementation plasmid pvra both before (−) and after (+) vancomycin induction. Ethidium bromide-stained 16S rRNA bands are shown below Northern blots as an indication of RNA loading. (B) Levels of luciferase activity from psas016p-luc+ in both uninduced and vancomycin-induced cultures of BB270 and its Δorf1, ΔYvqF, ΔVraS, and ΔVraR mutants. All values shown represent the means ± standard deviations (SD) obtained from two independent cultures. (C) Profiles of luciferase activity over growth in BB270ΔYvqF, BB270ΔVraS, and BB270ΔVraR, which contain psas016p-luc+. All values shown represent the means ± SD obtained from three independent cultures.