TABLE 2.
Primers used in this study
| Primer name and function | Nucleotide sequencea (5′-3′) | Source |
|---|---|---|
| Construction of pKOR1 mutagenesis plasmids | ||
| attB1-yvqF.upF | GGGGACAAGTTTGTACAAAAAAGCAGGCTGATTCCAAGTAAGCGTGTCAT | This study |
| attB2-yvqF.downR | GGGGACCACTTTGTACAAGAAAGCTGGGTCATCCATGTCATCCATAAGTA | This study |
| yvqF-XhoI.upR | ATTTACTCGAGATATTTGTGTGTCATGTTTCG | This study |
| XhoI-stop-yvqF.downF | AATTTCTCGAGTGATAGATATCAACGCAAATGTTGATC | This study |
| BamHI-orf1.upR | AATTTGGATCC CATAACTATCACCTT |
This study |
| BamHI-orf1.downF | AATTTGGATCCAATTATATGAACGCTGTGGCA | This study |
| XhoI-vraS.upR | ATTTACTCGAGGTTCATCGATAAATCACCTCTA | This study |
| XhoI-stop-VraS.downF | AATTTCTCGAGTGATAGCACTACATTAGAACAATTGGTT | This study |
| attB1-vraR.upF |
GGGGACAAGTT AAAAGCAGGCTGCTATTAATTGCATTCACATTAT |
This study |
| XhoI-vraR.upR | ATTTACTCGAGCGTCATACGAATCCTCCTTAT | This study |
| XhoI-stop-vraR.downF | AATTTCTCGAGTGATAGATTAAAGTATTGTTTGTGGAT | This study |
| attB2-vraR.downR |
GGGGACCACTT GAAAGCTGGGTAAACGAAGCTTAAGTCAGTATTA |
This study |
| Construction of complementation plasmids | ||
| vra.compF | AATTTGGATCCGCACATGTACTTAATTACTT | This study |
| vra.compR | AATTTGGTACCCGAATATGATGAAGATAGTA | This study |
| Construction of luciferase-fusion plasmid | ||
| SAS016.lucF | AATTA GGTACC TGGATCACGGTGCATACAAC | This study |
| SAS016.lucR | AATTA CCATGG CCTATATTACCTCCTTTGCT | This study |
| Amplification of DIG-labeled probes | ||
| vraR.For | ATGACGATTAAAGTATTGTT | This study |
| vraR.Rev | TTGAATTAAATTATGTTGGA | This study |
| SAS016.For | TCATACGTTCTATGTCTGAT | This study |
| SAS016.Rev | GATCTATATCGTCTTGTAAT | This study |
| Construction of BTH plasmids | ||
| yvqF.pKT25F | ATTAACTGCAGTGACACACAAATATATATCAA | This study |
| yvqF.pUT18CF | ATTAACTGCAGGACACACAAATATATATCAAC | This study |
| yvqF.BTHR | ATTAAGGTACCCGATAAATCACCTCTACGTC | This study |
| vraS.pKT25F | ATTAACTGCAGTGAACCACTACATTAGAACAA | This study |
| vraS.pUT18CF | ATTAACTGCAGGAACCACTACATTAGAACAAT | This study |
| vraS.BTHR | ATTAAGGTACCTCGTCATACGAATCCTCCTT | This study |
| vraR.pKT25F | ATTAACTGCAGTGACGATTAAAGTATTGTTT | This study |
| vraR.pUT18CF | ATTAACTGCAGGACGATTAAAGTATTGTTTG | This study |
| vraR.BTHR | ATTAAGGTACCTGAATTAAATTATGTTGGAA | This study |
| sigB.pKT25F | ATTAACTGCAGTGGCGAAAGAGTCGAAATCA | This study |
| sigB.pKT25R | ATTAAGGATCCTATTGATGTGCTCGTTCTTG | This study |
| rsbW.pUT18CF | ATTAACTGCAGGCAATCTAAAGAAGATTTTA | This study |
| rsbW.pUT18CR | ATTAAGGATCCTAGCTGATTTCGACTCTTTC | This study |
a
Restriction sites are underlined, attB sites are in boldface, and introduced stop codons are double underlined.