TABLE 5.
Comparison of MLST results obtained with two methodologies and spa typing
| LRSA isolate | PFGE type | MLST |
spa |
||
|---|---|---|---|---|---|
| PCR/ESI-MSa | Standard analysisb | Type | Repeats | ||
| M36 | A | CC5, USA100/800, ST5/ST225/ST371 | ST1788 | t1062 | 26-23-17-34-02-17-12-17-16 |
| I32 | A1 | CC5, USA100/800, ST5/ST225/ST371 | ST5 | t2 | 26-23-17-34-17-20-17-12-17-16 |
| M41 | A | CC5, USA100/800, ST5/ST225/ST371 | ST5 | t2 | 26-23-17-34-17-20-17-12-17-16 |
| M94 | A | CC5, USA100/800, ST5/ST225/ST371 | ST5 | t2051 | 26-23-17-34-17-20-16-12-17-16 |
| M86 | A3 | CC5, USA100/800, ST5/ST225/ST371 | ST5 | t105 | 26-23-17-34-17-20-17-17-16 |
| M92c | A4 | CC5, USA100/800, ST5/ST225/ST371 | ST5 | t45 | 26-17-20-17-12-17-16 |
| M32c | B | CC5, USA100/800, ST5/ST225/ST371 | ST5 | t7327 | 26-23-23-17-20-17-12-16 |
| M71c | A2 | CC5, USA100/800, ST5/ST225/ST371 | ST5 | t2115 | 26-23-17-13-17-20-17-17-16 |
a
All isolates were also confirmed to be S. aureus and were mecA positive, whereas genes conferring mupirocin resistance and production of toxins PVL, γ-hemolysin, and TSST-1 were not detected. PCR/ESI-MS was performed on a T5000 platform.
b
Allelic profile ST5 (arcC, allele 1; aroE, allele 4; glpF, allele 1; gmK, allele 4; pta, allele 12; tpi, allele 1; yqiL, allele 10) and ST1788 (arcC, allele 1; aroE, allele 4; glpF, allele 87; gmK, allele 4; pta, allele 12; tpi, allele 1; yqiL, allele 10).
c
These isolates were collected in 2009 from CF patients who were not included in the clinical statistical analysis covering the 7-year period (2000 to 2006).