FIG. 4.

Macromolecular synthesis. Incorporation of ³H-labeled radioactive isotopes was measured over time. (A) The CFU/ml was sampled at the same time isotope incorporation was measured. (B) Thymidine incorporation was measured to study the effects of Bac8c on DNA replication. Below the MBC, incorporation was unaffected. (C) Uridine incorporation was measured to study the effects of Bac8con transcription. Effects on transcription were seen at 40 μg/ml. (D) Histidine incorporation was measured to study the effects of Bac8c on translation. All concentrations of Bac8c had some effect on translation. In panels B to D, the y axis represents counts per minute (CPM) of radioactivity. Symbols: squares, control; diamonds, 2 μg/ml Bac8c; triangles, 4 μg/ml Bac8c; crosses, 40 μg/ml Bac8c (all in MOPS minimal medium). Cultures were started at an OD₆₀₀ of 0.2.