FIG. 6.
Methylglyoxal accumulation and hydroxyl radical formation. (A) Intracellular accumulation of MG occurs after 2 h of incubation with sublethal levels of Bac8c (sub-MIC level of Bac8c or 6 μg/ml; OD, 0.5). (B) Flow cytometry measurement of the increase in hydroxyl radical formation by the dye HPF after 2 h with control (filled light gray), cells exposed to the IC50 (3 μg/ml; dark gray), and cells exposed to the MBC (6 μg/ml; black). Hydroxyl radical formation after 60 min of exposure to Bac8c was not significantly different from the control. OD600, 0.1.